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Gomez Requeni, P; Calduch Giner, J; De Celis, S; Medale, Francoise; Kaushik, Sadasivam; Perez Sanchez, J. |
The activity of the somatotropic axis was analysed in juvenile rainbow trout (Oncorhynchus mykiss) fed either a fishmeal-based diet (FM) or graded levels of plant proteins to replace 50 % (PP50 diet), 75 % (PP75 diet) or 100 % (PP100 diet) of the fishmeal protein. For this purpose, partial cloning and sequencing of the gene encoding rainbow trout growth hormone receptor (GHR) was first accomplished by RT-PCR, using degenerate primers based on the sequences of non-salmonid fish GHR. Growth rates and energy retention were lowered by the PP75 and PP100 diets and a concurrent and progressive increase in plasma levels of growth hormone (GH) was found. However, no changes in hepatic GH binding and total plasma insulin-like growth factor (IGF)-I levels were... |
Tipo: Text |
Palavras-chave: Insulin like growth factor; Growth hormone receptor; Growth hormone; Plant proteins; Rainbow trout. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-3620.pdf |
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